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  Indian J Med Microbiol
 

Figure 4: Transcription kinetics of HPX family members under different pathophysiological conditions i.e. uninfected and Plasmodium vivax infected blood meal time series analysis: (A) Relative expression profiling of HPX family (hpx12,3,10,8 and hpx15, duox) members in blood meal time-series experiment; Salivary glands (SG) were collected from naive sugar-fed adult female mosquito and blood meal time series (6 h, 24 h, 48 h, 72h); (B) Transcriptional profiling of Hpx12 in SG in response to P. vivax infection (time point 8-10D,9-11D, and 12-14D) age-matched normal blood-fed SG was taken as a control; Three independent biological replicates (n=30, N3) were considered for statistical analysis viz. *p<0.05; **p<0.005 and ***p<0.0005 using Student’s t-test. (n=represents the number of mosquitoes pooled for sample collection; N= number of replicates).

Figure 4: Transcription kinetics of HPX family members under different pathophysiological conditions i.e. uninfected and <i>Plasmodium vivax</i> infected blood meal time series analysis: (A) Relative expression profiling of HPX family (hpx12,3,10,8 and hpx15, duox) members in blood meal time-series experiment; Salivary glands (SG) were collected from naive sugar-fed adult female mosquito and blood meal time series (6 h, 24 h, 48 h, 72h); (B) Transcriptional profiling of Hpx12 in SG in response to <i>P. vivax</i> infection (time point 8-10D,9-11D, and 12-14D) age-matched normal blood-fed SG was taken as a control; Three independent biological replicates (n=30, N3) were considered for statistical analysis viz. *p<0.05; **p<0.005 and ***p<0.0005 using Student’s t-test. (<i>n</i>=represents the number of mosquitoes pooled for sample collection; <i>N</i>= number of replicates).