Figure 3: Alteration of molecular and behavioral properties in AsHPX12 knock down mosquitoes. (A) The relative abundance of AsHPX12 mRNA in the salivary gland of control (dsLacZ) and (dsHPX-12) injected mosquitoes (p<0.002); (B) Comparative measurement of probing times among control and HPX12 knock down mosquitoes. The probing time is defined as the time taken from the initial insertion of the mouthpart into the skin until the initial observation of the ingestion of blood in the abdomen till full-fed. In each experimental set of mosquito group i.e., control and silenced mosquitoes (n=25) were offered blood meal, and the average time of fully fed mosquitoes was calculated. Each dot corresponds to accumulating mosquito group in a given time period. Probing times were significantly longer in HPX12 knockdown mosquitoes than in wild-type mosquitoes. The number and ratio of blood-fed mosquitoes within 200 seconds, significance calculate by non-parametric Wilcoxon-signed Rank test (p<0.001). (C) Comparative transcriptional profiling of the olfactory genes in the olfactory system (Consisting of Antenna, maxillary pulp and probosci’s) of the control vs HPX12 knock down mosquitoes. Transcript details are as follows: OBPs 10 (Odorant Binding Protein 10), OBP20, OBP7, OBP22, and OBP receptor IR75K (Ionotropic receptor 75K); (D) AsHPX12 knockdown mosquito showed upregulation of OBP10 and OBP20 gene compare to control mosquitoes, (E) The relative abundance of Apyrase mRNA in the salivary gland of control (dsLacZ) and knockdown (dsHPX-12) mosquitoes. (F) The relative abundance of Anophelin, 53.7kDa and SG2B mRNA in the salivary gland of control (dsLacZ) and knockdown (dsHPX-12) mosquitoes. Three independent biological replicates (n=30, N3) were considered for statistical analysis viz. *p<0.05; **p<0.005, ***p<0.0005 and NS-non significant using Student’s t-test. (n=represents the number of mosquito pooled for sample collection; N= number of replicates).