Figure 2: (A) Real-time PCR-based developmental expression analysis of AsHPX12 in An. stephensi mosquito aquatic stages L1 (larval stage1), L2 (larval stage2), L3 (larval stage3), L4 (larval stage 4), and whole body in male and female pupae; (B) Tissues specific expression kinetics of HPX family members in the naïve adult female mosquitoes: SG: Salivary glands; MG: Midgut; HC: Hemocytes, OV: Ovary; SP: Spermetheca. The ovary sample was considered as the control for the relative quantification of each test sample. Three independent biological replicates (n=30, N3) were considered for statistical analysis viz. *p<0.05; **p<0.005 and ***p<0.0005 using Student’s t-test. Final p-values were adjusted using Benjamini & Hochberg test. (n=represents the number of mosquitoes pooled for sample collection; N= number of replicates).