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  Indian J Med Microbiol
 

Figure 1: Genomic organization and molecular characterization of An. stephensi HPX-12: (A) Schematic representation of the genomic architecture of AsHPX12. Four brown color boxes (E1-E4) represent exons and +1 indicates the translation initiation site. A 50 bp UTR region is present on both 5’ and 3’ end of the transcript; (B) complete CDS sequence of identified AsHPX12 transcript: Nucleotide sequence has been shown as black alphabets, while encoded amino acid sequence has been below the respective triplicate genetic codes; dark blue underline remarks amino-acid sequence of the heme-peroxidase domain; light blue circle highlights the seven different motifs arrow marked to each of the motifs written in the right side of the circle; red and green colored underline nucleotide sequence marks the sequences selected for dsRNA primers and RT-PCR primer designing; green-colored dark arrow and red-colored dark arrow for forward/reverse primers highlights for forward/reverse primers); (C) Phylogenetic relationship of putative HPX12 homolog family proteins within the insect’s community: thirteen full-length top-hit BLASTp sequences were selected against AsHPX12 full-length amino-acid sequences and aligned through CLUSTALX program (Supplementary Fig. S7); the phylogenetic tree was generated through the MEGA program using the neighbor-joining method; (Species name and Accession IDs are mentioned). The red arrow indicates the location of the AsHPX12 sequence within Anopheles clades.

Figure 1: Genomic organization and molecular characterization of <i>An. stephensi HPX-12</i>: (A) Schematic representation of the genomic architecture of <i>AsHPX12.</i> Four brown color boxes (E1-E4) represent exons and +1 indicates the translation initiation site. A 50 bp UTR region is present on both 5’ and 3’ end of the transcript; (B) complete CDS sequence of identified <i>AsHPX12</i> transcript: Nucleotide sequence has been shown as black alphabets, while encoded amino acid sequence has been below the respective triplicate genetic codes; dark blue underline remarks amino-acid sequence of the heme-peroxidase domain; light blue circle highlights the seven different motifs arrow marked to each of the motifs written in the right side of the circle; red and green colored underline nucleotide sequence marks the sequences selected for dsRNA primers and RT-PCR primer designing; green-colored dark arrow and red-colored dark arrow for forward/reverse primers highlights for forward/reverse primers); (C) Phylogenetic relationship of putative HPX12 homolog family proteins within the insect’s community: thirteen full-length top-hit BLASTp sequences were selected against <i>AsHPX12</i> full-length amino-acid sequences and aligned through CLUSTALX program (Supplementary Fig. S7); the phylogenetic tree was generated through the MEGA program using the neighbor-joining method; (Species name and Accession IDs are mentioned). The red arrow indicates the location of the <i>AsHPX12</i> sequence within <i>Anopheles</i> clades.