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  Indian J Med Microbiol
 

Figure 5: PCR-RFLP genotyping profiles of Trypanosoma cruzi isolates obtained from mammals of the Eastern and Central-western regions/Venezuela. 2% agarose gel electrophoresis in TAE 1X. Each pair of lines corresponds to undigested PCR products (UD), followed by digested products (D) for HSP60/ EcoRV (A), GPI/HhaI (B), H1/AatII (C). From left to right: (M) Molecular Size marker 100 bp (Promega 100); (1-4) isolates from Didelphis marsupialis (DM), Cerdocyon thous (CTH); Desmodus rotundus (DES) from Eastern region; (5-10) isolates from DM from Central-western region.

Figure 5: PCR-RFLP genotyping profiles of <i>Trypanosoma cruzi</i> isolates obtained from mammals of the Eastern and Central-western regions/Venezuela. 2% agarose gel electrophoresis in TAE 1X. Each pair of lines corresponds to undigested PCR products (UD), followed by digested products (D) for HSP60/ EcoRV (A), GPI/HhaI (B), H1/AatII (C). From left to right: (M) Molecular Size marker 100 bp (Promega 100); (1-4) isolates from <i>Didelphis marsupialis</i> (DM), <i>Cerdocyon thous</i> (CTH); <i>Desmodus rotundus</i> (DES) from Eastern region; (5-10) isolates from DM from Central-western region.