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  Indian J Med Microbiol
 

Figure 1: Amplification of the non-transcribed spacer of the mini-exon gene of Trypanosoma cruzi isolates from triatomes and mammals of the Eastern and Central-Western regions/Venezuela. Agarose gel electrophoresis (2%). TcI: 200 base pairs (bp) band, TcIV: 250 bp band; TcIII: 150 bp band. (a) Triatomines: (M) Molecular Size marker-100 bp (Promega); (1) + Control TcI (EP strain, from a patient in acute stage of Chagas disease); (2) + Control TcIV (MA286XC Strain, from Dasypus novemcintus in Paraguay); (3) + Control TcIII (Cachi1 Strain, from D. novemcintus Anzoátegui-Venezuela); (4-8) Isolates from Triatoma maculata (TM); Panstrongylus geniculatus (PG); Psammolestes arthuri (PA) from Eastern region; (9-13) Isolates from TM; PG; Rhodnius prolixus (RP) from Central-Western region. (b) Mammals: (4-8) Isolates from Didelphis marsupialis (DM), Cerdocyon thous (CTH); Odocoileus margaritae (ODM) from Eastern region; (9-12) Isolates from DM from Central-Western region. C – negative control.

Figure 1: Amplification of the non-transcribed spacer of the mini-exon gene of <i>Trypanosoma cruzi</i> isolates from triatomes and mammals of the Eastern and Central-Western regions/Venezuela. Agarose gel electrophoresis (2%). TcI: 200 base pairs (bp) band, TcIV: 250 bp band; TcIII: 150 bp band. (a) Triatomines: (M) Molecular Size marker-100 bp (Promega); (1) + Control TcI (EP strain, from a patient in acute stage of Chagas disease); (2) + Control TcIV (MA286XC Strain, from <i>Dasypus novemcintus</i> in Paraguay); (3) + Control TcIII (Cachi1 Strain, from <i>D. novemcintus</i> Anzoátegui-Venezuela); (4-8) Isolates from <i>Triatoma maculata</i> (TM); <i>Panstrongylus geniculatus</i> (PG); <i>Psammolestes arthuri</i> (PA) from Eastern region; (9-13) Isolates from TM; PG; <i>Rhodnius prolixus</i> (RP) from Central-Western region. (b) Mammals: (4-8) Isolates from <i>Didelphis marsupialis</i> (DM), <i>Cerdocyon thous</i> (CTH); <i>Odocoileus margaritae</i> (ODM) from Eastern region; (9-12) Isolates from DM from Central-Western region. C – negative control.