Close
  Indian J Med Microbiol
 

Figure 3: Ethidium bromide stained 3% agarose gel showing the restriction enzyme digested amplified products in three common G6PD mutations. Lane 1 sample lacks the digestion site and produced—(a) 377 and 198 bp products in exon 6/7 with MboII; (b) 107, 75 and 66 bp products with HaeIII; and (c) 74 and 60 bp products with MnlI restriction enzymes respectively. However, Lane 2 (Control) produced—(a) 277, 119 and 100 bp products in exon 6/7; (b) 123, 107 and 66 bp products in exon 3/4; and (c) 80 and 60 bp products in exon 9 upon digestion. M1, M2 and M3—Molecular markers of size 100, 50 and 20 bp respectively; U—Undigested product [575 bp in (a), 352 bp in (b), and 275 bp in (c)]; 1—Patient sample DNA; 2—G6PD deficient controls.

Figure 3: Ethidium bromide stained 3% agarose gel showing the restriction enzyme digested amplified products in three common G6PD mutations. Lane 1 sample lacks the digestion site and produced—(a) 377 and 198 bp products in exon 6/7 with <i>MboII</i>; (b) 107, 75 and 66 bp products with <i>HaeIII</i>; and (c) 74 and 60 bp products with <i>MnlI</i> restriction enzymes respectively. However, Lane 2 (Control) produced—(a) 277, 119 and 100 bp products in exon 6/7; (b) 123, 107 and 66 bp products in exon 3/4; and (c) 80 and 60 bp products in exon 9 upon digestion. M1, M2 and M3—Molecular markers of size 100, 50 and 20 bp respectively; U—Undigested product [575 bp in (a), 352 bp in (b), and 275 bp in (c)]; 1—Patient sample DNA; 2—G6PD deficient controls.