Journal of Vector Borne Diseases

RESEARCH ARTICLE
Year
: 2022  |  Volume : 59  |  Issue : 3  |  Page : 206--215

Salivary AsHPX12 influences pre-blood meal associated behavioral properties in Anopheles stephensi


Seena Kumari1, Tanwee Das De1, Charu Chauhan1, Jyoti Rani1, Sanjay Tevatiya1, Punita Sharma1, Veena Pande2, Rajnikant Dixit1 
1 Laboratory of Host-Parasite Interaction Studies, ICMR-National Institute of Malaria Research, New Delhi, India
2 Department of Biotechnology, Kumaun University, Uttarakhand, India

Correspondence Address:
Dr Rajnikant Dixit
Laboratory of Host-Parasite Interaction Studies, ICMR-National Institute of Malaria Research, Dwarka, New Delhi
India

Background & objectives: A successful blood meal acquisition process by an adult female mosquito is accomplished through salivary glands, which releases a cocktail of proteins to counteract the vertebrate host’s immune homeostasis. Here, we characterize a salivary-specific Heme peroxidase family member HPX12, originally identified from Plasmodium vivax infected salivary RNAseq data of the mosquito Anopheles stephensi. Methods: To demonstrate we utilized a comprehensive in silico and functional genomics approach. Results: Our dsRNA-mediated silencing experiments demonstrate that salivary AsHPX12 may regulate pre-blood meal-associated behavioral properties such as probing time, probing propensity, and host attraction. Altered expression of the salivary secretory and antennal proteins expression may have accounted for salivary homeostasis disruption resulting in the unusual fast release of salivary cocktail proteins and delayed acquisition of blood meal in the AsHPX12 knockdown mosquitoes. We also observed a significant parallel transcriptional modulation in response to blood feeding and P. vivax infection. Interpretation & conclusion: With this work, we establish a possible functional correlation of AsHPX12 role in the maintenance of salivary physiological-homeostasis, and Plasmodium sporozoites survival/transmission, though the mechanism is yet to unravel.


How to cite this article:
Kumari S, De TD, Chauhan C, Rani J, Tevatiya S, Sharma P, Pande V, Dixit R. Salivary AsHPX12 influences pre-blood meal associated behavioral properties in Anopheles stephensi.J Vector Borne Dis 2022;59:206-215


How to cite this URL:
Kumari S, De TD, Chauhan C, Rani J, Tevatiya S, Sharma P, Pande V, Dixit R. Salivary AsHPX12 influences pre-blood meal associated behavioral properties in Anopheles stephensi. J Vector Borne Dis [serial online] 2022 [cited 2023 Feb 2 ];59:206-215
Available from: http://www.jvbd.org//article.asp?issn=0972-9062;year=2022;volume=59;issue=3;spage=206;epage=215;aulast=Kumari;type=0