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The Relationship between MMP2 rs243865, MMP-9 rs398242and CXCL-12 rs1801157 gene Polymorphisms with Japanese encephalitis disease and disease outcome in North Indian population
Rashmi Tiwari1, Sneha Ghildiyal2, Pooja Gaur2, Tanzeem Fatima2, Shivbrat Upadhyay3, Janmejai K Srivastva4, Virendra Atam5, Tapan N Dhole2
1 Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow-226014, Uttar Pradesh; Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow-226028, India
2 Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow-226014, Uttar Pradesh, India
3 Era's Lucknow Medical College and Hospital, Lucknow-226003, Uttar Pradesh, India
4 Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow-226028, India
5 King George Medical University, Lucknow-226003, Uttar Pradesh, India
Correspondence Address:
Janmejai K Srivastva,
Director and Head, Amity Institute of Biotechnology, Amity University, Lucknow, Uttar Pradesh, India.
India
 Source of Support: None, Conflict of Interest: None DOI: 10.4103/0972-9062.361176
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Background & objectives: Japanese encephalitis virus (JEV) is one of the most important causes of acute and uncontrolled inflammatory disease in Asia. MMPs and chemokines are play a detrimental role in the host response to JE disease, aetiology, and disease outcome. Evidently, MMPs are widely circulated in the brain and regulate various process including microglial activation, inflammation, blood-brain barrier disruption as well as affects central nervous system (CNS). The present study was to assess the association of single nucleotide polymorphisms of MMP-2, MMP-9 and chemokine (CXCL-12/SDF’) in north Indian population.
Methods: We performed case-control study and comprised125 patients and 125 healthy controls in north Indian population. Genomic DNA was extracted from whole blood and gene polymorphism have been determined by PCR-RFLP method.
Results: MMP-2, MMP-9 and CXCL-12 gene was not significantly associated with JE disease, but homozygous (T/T) genotype of MMP-2 was statically associated with disease outcome (p=0.05, OR=0.110). A/G and G/G genotype of CXCL-12 was significantly associated with severity of disease. (p=0.032, OR=5.500, p=0.037, OR= 9.167). The serum level of MMP-2 was observed significantly increased in JE patients with homozygous (G/G) genotype whereas increased MMP-9levelwas associated with heterozygous genotype.
Interpretation &conclusion: MMP-2, MMP-9 and CXCL-12 gene polymorphism were not associated with JE susceptibility, but MMP-2 may be contributed to disease protection. CXCL-12 was associated with disease severity. In our concern this is the first report from northern India.
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