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Potential Larvicidal Bioefficacy of Copper Sulfide Based Hybrid Nanoemulsions of Eucalyptus Oil against Aedes aegypti (Linnaeus)

1 Department of Zoology, Punjab Agricultural University, Ludhiana-141004; Akal University, Talwandi Sabo, Bathinda-151302, Punjab, India
2 Department of Zoology, Punjab Agricultural University, Ludhiana-141004, Punjab, India

Correspondence Address:
Komalpreet Kaur Sandhu,
Assistant Professor, Department of Zoology, Akal University, Talwandi Sabo, Punjab-151302, India.
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-9062.361167

Background ' objectives: Nanotechnology, an emerging field has acquired considerable attention for the control of vectors. The present study aimed to synthesize, characterize Copper sulfide and eucalyptus oil based hybrid nanoemulsions and investigate their larvicidal potential against Aedes aegypti by studying larvicidal bioassay, morphological aberrations, histopathological alterations, biochemical analysis and evaluation of risk assessment in non-target organisms. Methods: Hybrid nanoemulsions were prepared by mixing aqueous Copper sulfide nanoparticles (CuSNPs) with non polar eucalyptus oil in five ratios (1:1, 1:2, 1:3, 1:4 and 1:5) by sonication, screened and characterized using TEM. Larvicidal activity was recorded and toxicity values were calculated by log-probit method. Morphological, histological and biochemical changes were examined in Aedes aegypti larvae after treatment. Nanohybrids were also tested under simulated conditions and against non-target organism. Results: The nanohybrid ratio of 1:5 was found to be stable after thermodynamic stability tests. TEM studies revealed average size of 90±7.90 nm with globular shape. LC50 and LC90 toxicity values of prepared CuSNPs were calculated out to be 5.00 and 5.81ppm after 24 hours treatment. Effective concentration of prepared nanohybrid (6.5ppm) tested under simulated conditions showed maximum larvicidal mortality after 48 hrs of exposure. No toxicity towards the Mesocyclops spp. was observed after treatment of these nanohybrids even up to 21 days. Interpretation & conclusion: Copper sulfide based hybrid nanoemulsions were found to show efficient larvicidal property which can be used for the formulation of ecofriendly bio-larvicide against Aedes aegypti.

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