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Investigation of Chikungunya virus Genotype at tertiary care center in Western Maharashtra
Kalpana Tikaram Suryavanshi, Chakraborty Ankana
Department of Microbiology, Bharati Vidyapeeth Deemed To Be University & Medical College, Pune, India
Correspondence Address:
Kalpana Tikaram Suryavanshi,
Department of Microbiology, Bharati Vidyapeeth Deemed To Be University & Medical College, Pune
India
 Source of Support: None, Conflict of Interest: None DOI: 10.4103/0972-9062.353231
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Background & objectives: Chikungunya is re-emerging arbovirus infection. Laboratory diagnosis can be done by Classical test involving Rapid Immunochromatography, Enzyme Linked Immunosorbent assay & by Molecular methods. Present study was undertaken to know the Genotype of Chikungunya virus among patients suspected of CHICKV & Investigated by virus culture, Partial sequencing, Rapid Immunochromatography, Enzyme linked Immunosorbent assay (ELISA). To understand different techniques used in Chikungunya diagnosis viz, virus culture, Partial sequencing along with Immunochromatography & ELISA.
Methods: Prospective, Laboratory based study at tertiary care centre. Lateral flow chromatography & ELISA was carried out on serum samples. All the 50 samples were cultured & indirect Immunofluorescence was performed on positive samples at Interactive Research School for Health affairs (IRSHA), at Bharati Vidyapeeth Medical College Pune, Maharashtra.
Virus isolates were subjected to partial sequencing for identification of genotype after confirmation by PCR. Stastical package of Social Science (SPSS) version 22.0 software was used to calculate Receiver operating curve (ROC) for different tests.
Results: Out of fifty samples, twenty were positive by Immunochromatography, 23 by ELISA, 3 by culture, PCR confirmed CHIKV isolates & sequencing identified genotypes as East Central South African type.
Interpretation & conclusion: CHIKV culture isolates of East Central South African type linage were predominantly found in present study. These are also common genotype present in Asia & India. |
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