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A Multiplex PCR assay for a differential diagnostic of rickettsiosis, Lyme disease and scrub typhus
Gaspar Peniche-Lara1, Isamara Moo-Salazar1, Karla Dzul-Rosado2
1 Laboratorio de Enfermedades Infecciosas y Parasitarias 1. Unidad Interinstitucional de Investigación Clínica y Epidemiológica, Facultad de Medicina, Universidad Autónoma de Yucatán. Yucatán, Mexico
2 Laboratorio de Enfermedades Emergentes y Re-emergentes. Centro de Investigaciones Regionales "Dr. Hideyo Noguchi". Universidad Autonoma de Yucatan. Yucatán, Mexico
Correspondence Address:
Gaspar Peniche-Lara,
Unidad Interinstitucional de Investigación Clinica y Epidemiologica, Facultad de Medicina, Universidad Autonoma de Yucatan, Avenida Itzaes por 58, Centro, 97000, Merida, Yucatan
Mexico
 Source of Support: None, Conflict of Interest: None DOI: 10.4103/0972-9062.337506
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Background & objectives: Coexistence of tick-borne diseases in some regions in Latin America makes difficult the diagnostic due to shares initial signs and symptoms. Rickettsiosis, Lyme disease and recently scrub typhus makes are gaining more importance in recent years. The objective of the study is developing a multiplex-PCR assay for a differential diagnosis of rickettsiosis, Lyme disease and scrub typhus.
Methods: By using bibliographic and bioinformatic analysis, we identify candidate regions to perform the multiplex-PCR assay for Rickettsia sp. Borreliaburgdorferi and Orientia tsutsugamushi as well as the identification of the optimal melting temperature and the sensibility analysis
Results: We identificate specific primer pairs for Rickettsia sp, Borreliaburgdorferi and Orientia tsutsugamushi with different PCR fragment length but a common melting temperature, 58C.
Interpretation & conclusion: We successfully develop a Multiplex PCR assay for a differential diagnosis of rickettsiosis, Lyme disease and scrub typhus that could be a rapid and easy option in the clinical an epidemiological practice. |
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