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Co-distribution of Dengue and Chikungunya viruses in Aedes mosquitoes of Delhi, India

1 ICMR-National Institute of Malaria Research, New Delhi, India
2 South Delhi Municipal Corporation, Delhi, India

Correspondence Address:
Rekha Saxena,
Scientist- F, ICMR-NIMR, Sector-8, Dwarka, New Delhi - 110077
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-9062.325638

Background & objectives: Dengue and chikungunya are vector borne diseases of great public health concern in India. Dengue outbreaks were recorded over the last few decades from almost all parts of India. In 2016, 101388 dengue cases and 210 deaths were reported in India including 4337 cases and 6 deaths from Delhi, India whereas Chikungunya outbreaks were reported from different states with 1.3 million cases in 2006. Dengue virus (DENV) and Chikungunya virus (CHIKV) are transmitted by the same species of Aedes mosquito. DENV and CHIKV co-infections have been reported from 13 of 98 countries with local transmission for both the diseases. The reasons for the sudden upsurge of cases of these diseases are poorly understood. Methods: A study was conducted in 66 localities of Delhi in collaboration with Municipal Corporation of Delhi from March-December 2016. Localities were selected on the basis of confirmed dengue cases reported during the last five years and study area was visited once every month. A door-to-door entomological survey was carried out to find the Aedes breeding in each water filled containers present in and around houses. Both immature and mature stages of Aedes mosquitoes were collected. Mosquitoes were pooled (n≤10 each for male and female) breeding site-wise and stored in Trizol at -80°C. Muliplex RT-PCR was performed for Chikungunya and dengue virus detection. Results: A total 981 Aedes mosquitoes were distributed in 146 Pools and were tested for DENV and CHIKV using Multiplex Reverse Transcriptase-PCR. The presence of Chikungunya virus was detected in 19 pools whereas 8 pools of females captured adults were found positive for dengue virus. No co-infection was observed in any pool. Interpretation & conclusion: Continuous surveillance for both dengue and Chikungunya viruses is essential in the endemic areas for identification and characterization of these viral pathogens. This information will also help in the implementation of proper measures to control the outbreaks caused by these emerging viral pathogens.

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