• Users Online: 872
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
RESEARCH ARTICLE
Year : 2022  |  Volume : 59  |  Issue : 2  |  Page : 154-162

In silico study to predict promiscuous T cell and B cell-epitopes derived from the vaccine candidate antigens of Plasmodium vivax binding to MHC class-II alleles


1 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra, Kingdom of Saudi Arabia
2 Universal Group of Institutions, Lalru, Punjab, India
3 Department of Basic Oral Medicine and Allied Dentistry, College of Dentistry, Taif University, Taif, Kingdom of Saudi Arabia
4 Postgraduate Institute of Medical Education and Research, Chandigarh, India
5 Department of Basic Oral Medicine and Allied Dentistry, College of Dentistry, Taif University, Taif, Kingdom of Saudi Arabia; Faculty of Biotechnology and Applied Sciences, Shoolini University, Himachal Pradesh, India

Correspondence Address:
Umar Farooq
Department of Basic Oral Medicine and Allied Dentistry, College of Dentistry, Taif University, Taif, KSA; Faculty of Biotechnology and Applied Sciences, Shoolini University, Solan, HP, India

Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-9062.335726

Rights and Permissions

Malaria is one of the major causes of health and disability globally, even after tremendous efforts to eradicate it. Till date no highly effective vaccine is available for its control. The primary reason for the low efficacy of vaccines is extensive polymorphism in potential vaccine candidate antigen genes and HLA polymorphisms in the human population. This problem can be resolved by developing a vaccine using promiscuous peptides to combine the number of HLA alleles. This study predicted T and B cell epitopes (promiscuous peptides) by targeting PPPK-DHPS and DHFR-TS proteins of Plasmodium vivax, using different in silico tools. Selected peptides were characterized as promiscuous peptides on the basis of their immunogenicity, antigenicity and hydrophobicity. Furthermore, to confirm their immunogenicity, these peptides were utilized for molecular modelling and docking analysis. For determining the requisite affinity with distinct HLA Class-I, and HLA Class-II alleles, only five peptides for DHFR-TS and 3 peptides for PPPK-DHPS were chosen as promiscuous peptides. The D1 peptide has the maximum binding energy with HLA alleles, according to HLA-peptide complex modelling and binding interaction analyses. These findings could lead to the development of epitope-based vaccinations with improved safety and efficacy. These epitopes could be major vaccine targets in P. vivax as they possess a higher number of promiscuous peptides. Also, the B cell epitopes possess maximum affinity towards different alleles as analyzed by docking scores. However, further investigation is warranted in vitro and in vivo.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed809    
    Printed10    
    Emailed0    
    PDF Downloaded34    
    Comments [Add]    

Recommend this journal