Serological and molecular survey of zoonotic visceral leishmaniasis in stray dogs (Canis familiaris) from an endemic focus in Meshkin-Shahr district in Ardabil province, Iran
Fatemeh Tabatabaie1, Yashar Nasirikaleybar1, Mehdi Mohebali2, Rahmat Solgi3, Vahid Babaei4, Zahra Heidari5, Fariba Orujzadeh1, Zabihollah Zarei2
1 Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2 Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3 Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
4 Genetic department, Iran University of Medical Sciences, Tehran, Iran
5 Department of Medical Microbiology and Parasitology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
Department of Parasitology andMycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran
Source of Support: None, Conflict of Interest: None
Background & objectives: Visceral leishmaniasis (VL),a protozoan disease caused by Leishmania infantum is a major public health problem and cause of death among infants aged under 1 year and the elderly in endemic foci of Iran. The aim of this study is to determine the status of L.infantum infection in stray dogs from Meshkin-Shahr, a typical endemic area of VL in Iran.
Methods: Sixty-eight randomly trapped stray dogs in Meshkin-Shahr area were tested for L. infantum infection using the direct agglutination test (DAT) from June to October 2016. The confirmation of seropositive samples was performed by Microscopic slides of spleen, culture and then PCR. The molecular methods performed by ITS1-PCR, RFLP-PCR and kDNA-PCR. The allof kDNA -PCR products were sequenced.
Results: Out of 68 examined stray dogs, 17 (25.0%) were positive for L. infantum by DAT (1:320 titers or higher). Parasite test showed that all of seropositive samples have amastigote forms in their spleens but only 3 out of them could be cultured. The kDNA-PCR confirmed all of seropositive samples but ITS1-PCR and RFLP-PCR only confirmed 3 out of 17 (17.6%) seropositive samples. The sequenced products showed 94% homology with L. infantum.
Interpretation & conclusion: The results showed a high prevalence of L. infantum infection in dogs in an endemic area of CVL and it provided key information for designing control programs against canine and human leishmaniasis.