|Year : 2021 | Volume
| Issue : 2 | Page : 115-118
First molecular survey of Anaplasma phagocytophilum in hard ticks (Ixodidae) from Southwestern Iran
Hossein Hamidinejat1, Mohammad HosseinRazi Jallali1, Somayeh Bahrami1, Mohammad Bagheri2
1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Ms.C of Parasitology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
|Date of Submission||09-Sep-2018|
|Date of Decision||28-Dec-2020|
|Date of Web Publication||13-Jan-2022|
Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz
Source of Support: None, Conflict of Interest: None
Anaplasma phagocytophilum is a gram-negative obligate intracellular tick-borne rickettsia with veterinary and public health importance worldwide. This organism is an etiologic agent of tick-borne fever (TBF) in domesticated animals and human granulocytic anaplasmosis (HGA) as well. Hard ticks (Ixodida: Ixodidae) are incriminated as the main biologic vectors for Anaplasma spp. Studies represent that Ixodes spp. are the main vectors for A. phago-cytophilum and few reports hinted that other tick species may play this role. So, the goal of the presented work was to investigate the A. phagocytophilum in 2000 hard ticks in Khuzestan province of Iran by specific nested-PCR performing two consecutive amplifications of 16SrRNA gene fragment with highly variable nucleotide region. Each reaction included 10 salivary glands of distinct tick species. Specific nested-PCR on accumulated salivary glands detected specific bands in 15.5% of reactions (31 of 200) in electrophoresis only in Rhipicephalus sanguineous and Hyalomma marginatum ticks. We concluded that the broad distribution of A. phagocytophilum infection is not only is due to the existence of Ixodes spp. but other hard ticks may also play a role in this issue.
Keywords: Anaplasma phagocytophilum; Hard ticks; Nested-PCR; Iran
|How to cite this article:|
Hamidinejat H, Jallali MH, Bahrami S, Bagheri M. First molecular survey of Anaplasma phagocytophilum in hard ticks (Ixodidae) from Southwestern Iran. J Vector Borne Dis 2021;58:115-8
|How to cite this URL:|
Hamidinejat H, Jallali MH, Bahrami S, Bagheri M. First molecular survey of Anaplasma phagocytophilum in hard ticks (Ixodidae) from Southwestern Iran. J Vector Borne Dis [serial online] 2021 [cited 2022 Jan 29];58:115-8. Available from: https://www.jvbd.org/text.asp?2021/58/2/115/316273
| Introduction|| |
Hard ticks (Ixodida: Ixodidae) are responsible for the transmission of many microbial infections due to its sanguinary biology. In Middle East countries, ticks and tick-borne diseases are considerably prevalent because of the modality of the tropical and sub-tropical nature. Many factors, for instance, numerous host species, the general condition of the hosts, and genetic diversities among the pathogenic organisms influence the ambiguous distribution of tick-borne diseases and make them intricate to control. Anaplasma phagocytophilum (Rickettsiales: Anaplasmataceae) is an emerging zoonotic tick-borne rickettsia, with veterinary and public health importance worldwide,. This gram-negative obligate intracellular bacterium is well known as the etiologic agent of tick-borne fever (TBF) in domesticated animals including ruminants, dogs, and horses and human granulocytic anaplasmosis (HGA). Several wild animals are also infected with this rickettsia. This broad spectrum of reservoirs makes the organism with a spacious distribution pattern in mammalians including humans,. Recent reports increasingly imply a worldwide prevalence of A. phagocytophilum in mentioned hosts. Accordingly, A. phagocytophilum is reported hitherto from mammals of all European countries and also the United States. In Iran, there are no available confirmative data or reports about human A. phagocytophilum infection but our previous surveys confirmed this organism in dogs and ruminants,,.
There is no confirmed detection of A. phagocytophilum in the salivary gland of vector ticks except the tracing DNA of this rickettsia. For this reason, among the different targeted genes for amplification in PCR, 16SrRNA gene fragment with highly variable nucleotide region appears more reliable for A. phagocytophilum detection. Literature predominantly represented that A. phagocytophilum is transmitted by Ixodes spp. and there are few reports hinted that other ticks may also act as vectors. Our molecular laboratory observations revealed that despite the absence of Ixodes spp. in the region of study, this bacterium is present in dogs, cows, and camels. Therefore, we aimed to investigate the existence of A. phagocytophilum and the probability of the circulating bacterium in salivary glands of predominant hard ticks in the Khuzestan province of Iran by a precise molecular method, i.e. specific nested PCR.
| Material & Methods|| |
From January 2015 to June 2017, total 2000 of pre-dominant hard ticks, including 1000 Rhipicephalus sanguineous, 500 Hyalomma anatolicum anatolicum, and 500 H. marginatum were collected from cattle and sheep reared in different districts of Khuzestan province of Iran with 64.055 km2 area. The identification of selected ticks was done using the identification key published previously. Afterward, individual ticks were dissected and their salivary glands were removed. Each 10 collected salivary glands from distinct tick species were pooled and preserved immediately in a tube with one ml of 70% ethanol until later molecular examination. Therefore, each positive or negative reaction in the molecular examination was representative of 10 accumulated salivary gland samples from a distinct tick species.
DNA extraction, PCR, nested PCR, and specific nested PCR
DNA was exploited by the application of the genomic DNA extraction Kit (Cinnagen, Iran). Species specification was accomplished by specific nested PCR according to amplification of the 16SrRNA gene which conserved for all Anaplasma species. PCR protocol and primer selection were adopted according to the previously described. Briefly, amplification of the 16S rRNA gene was performed in 25μl reaction volumes including 5μl of DNA template, 5 pmol of forward and reverse primers (each 1μl), 12.5μl of master mix (Ampliqon, Denmark) containing 3mM MgCl2, 0.4mM of each dATP, dCTP, dGTP and dTTP and 0.08 U/ml Taq DNA polymerase in reaction buffer. PCR reactions included a negative control consisting of the reaction mix and 2 μl of DNase/ RNase-free water instead of DNA. Positive control consists of a DNA sample from the ticks that their infection to the A. phagocytophilum was confirmed with sequencing (accession number: MN795150). Forward and reverse genus-specific primer sequences were 5′AGAGTTTGATCCTGGCTCAG3′ and 5′AGCACTCATCGTTTACAGCG3′, respectively. The thermal cycles of PCR were as follows: 95°C for 5 min, 35 cycles of 94°C for 45 sec, annealing at 56°C for 45 sec, and 72°C for 45 sec, followed by a final extension step at 72°C for 5 min. Amplified products were identified using 2% of agarose gel stained by safe stain and compared with a 100bp ladder after visualization by UV transilluminator. Later stages were carried out on products of prime PCR by forward and reverse primers with 5′GCAAGCTTAACACATGCAAGTA 3′ and 5′ GTTAAGCCCTGGTATTTCAC 3′ for nested PCR to confirm the Anaplasma spp. and 5′ CTTTATAGCTTGCTATAAAGAA 3′ and 5′ GTTAAGCCCTGGTATTTCAC 3′ for specific nested PCR to confirm A. phagocytophilum. All of the circumstances for nested PCR and specific nested PCR including thermal program and detecting the products were identical to prime PCR.
| Results|| |
In total, 31 samples of the approximately 781 bp length bands achieved by amplification of 16S rRNA gene of Anaplasma spp. in prime PCR [Figure 1]A were confirmed by detection of expected 544 bases pare length bands during the first nested-PCR [Figure 1]B and 509 bp length bands by subsequent specific nested-PCR [Figure 1]C. Specific nested-PCR on 200 accumulated salivary glands (each reaction includes 10 salivary glands of 10 distinct tick species) revealed that specific bands with 509 bp length bands were exhibited in 15.5% of cases (31 of 200) in electrophoresis. Positive results were relevant to R. sanguineous and H. marginatum and all of the stacked salivary glands from H. anatolicum anatolicum were negative for A. phagocytophilum infection; 21 of 50 pooled samples of H. marginatum and 10 of 100 pooled samples of R. sanguineous were clearly infected with this organism.
|Figure 1: Agarose gel electrophoresis of PCR of the 16SrRNA gene (A), Nested PCR (B), and Specific Nested PCR (C) products. A. 1-6: 781 bp of amplified products of Anaplasma spp. B. 1-10: 543 bp of amplified products Anaplasma spp.in nested-PCR. C. 1-10: 509 bp of amplified products of A. phagocytophilum. P= Positive control. N= Negative control.|
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| Discussion|| |
Anaplasmosis is an important issue for animal breeders in terms of economic losses as well as a health concern to humans. Ticks are considered as the main vector of the disease. The previous investigation showed that Ornithodoros lahorensis, H. dromedarii, H. anatolicum, H. asiaticum, H. marginatum, H. detritum, R. sanguineus, Dermacentor marginatus, D. niveus, and Argas persicus are considered as potential vectors for Anaplasma spp. in Iran. Another study showed that R. sanguineus, R. bursa, Haemaphysalis punctata, and D. marginatus are the main vectors for A. ovis in Iran. Although different studies showed the potential vectors for Anaplasma spp. in Iran there is very limited information about the possible vectors of A. phagocytophilum.
Our observation on successively achieved 781, 544 and 509 bp bands in gel electrophoresis following specific nested-PCR convinced that A. phagocytophilum infection was present among examined ticks, more in H. marginatum and less in R. sanguineous. Sampling was done from January till June in which hard ticks were at their annual peak in Khuzestan province in Iran. This region is enzootic for some veterinary tick-borne diseases, including theileriosis, babesiosis, and also anaplasmosis due to A. marginale. The outbreak of these diseases commonly occurs in later spring till mid summer,,,.
Our previous investigation showed that Iranian camels can be a reservoir of A. phagocytophilum and was found to be enzootic, as indicated by the high subclinical infection rate. In Khuzestan province where the current study is carried out, we confirmed that the molecular prevalence of A. phagocytophilum was noticeably high in dogs. Furthermore, we showed that 7.5% of cattle in Khuzestan province were infected with both A. phagocytophilum and A. marginale. We claimed that despite the healthy appearance of infected cattle, they may transmit Anaplasma to ticks and are potential continuous sources for maintaining and disseminating the organisms to the human and animals’ population.
Although most literature, but not all, suggests that predominantly Ixodes scapularis and I. ricinus tick species are the main vectors of A. phagocytophilum,, we confirmed again that other genera of hard ticks may act as potentially active vectors. Many reports relevant to Ixodes spp. as vectors of A. phagocytophilum are from Europe in which this tick is prevalent. Only in limited areas of Iran, far from the zone of sampling, Ixodes ricinus is present and there is no report that conveys the existence of I. scapularis or any other species of this genus. However, these areas are completely isolated by seamless mountains and there is no livestock exchange with the district of presented work. Except for Ixodes spp., this bacterium has been recognized by molecular approaches in different ticks from different countries, for instance, in H. longicornis, H. concinna, H. qinghaiensis, and D. silvarum in China. Also, it was revealed that 13.7% of harvested R. sanguineous from dogs in Giza, Egypt were positive for A. phagocytophilum utilizing sequence analysis of the 16S rRNA gene and confirmed this tick species as a vector of this rickettsia. Their report is confirmatory for our results. Worldwide distribution and considerable prevalence of tick-borne diseases such as TBF in domesticated animals and HGA in human populations indicate a wide range and also well-distributed tick vectors. It was revealed that Ixodes species are potentially active vectors of A. phagocytophilum in the northern hemisphere, especially in temperate zones. Just in Norway, the existence of A. phagocytophilum in host-seeking I. ricinus ranges from 1.4 % to 19.4 %,. However, the scenario may be different in tropics and sub-tropics when this hard tick genus is absent.
The DNA of A. phagocytophilum was present in ticks of relatively all of the districts where we considered for sampling including boundaries of the province. Many ranchers of Khuzestan province have transhumance living in which they relocate to neighboring provinces with their livestock, mainly sheep and goats, on a seasonal basis. This lifestyle is traditional for the locals, but it, unfortunately, breaks the quarantine and makes the boundaries of the province unstable enzootic sites, where the naive animals experience an acute form of the diseases. The main problems and fatal cases of TBF are in animals that encounter the tick and rickettsia for the first time. The first report of A. phagocytophilum infection was documented in two cattle by specific nested-PCR in the neighborhood of lodgment of nomadic tribes from Isfahan province in central Iran.
Anaplasma phagocytophilum infection is an emerging disease with increasing reports in both animals and humans all around the world. This broad distribution in infection is maintained by different potentially active vectors, maybe more than one genus of hard ticks. Here, we determined that A. phagocytophilum DNA existed in salivary glands of two predominant ixodid ticks, H. marginatum and R. sanguineous in Khuzestan province from Iran. In future studies, we will focus on A. phagocytophilum isolation from ticks and molecular identification and genetic analysis of isolated strains.
Conflicts of interest: None
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