| RESEARCH ARTICLE |
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| Year : 2013 | Volume
: 50
| Issue : 3 | Page : 197-201 |
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Genotyping of MSP3β gene in Indian Plasmodium vivax
Purva Gupta1, Veena Pande2, Alex Eapen3, Vineeta Singh1
1 National Institute of Malaria Research, New Delhi, India
2 Department of Biotechnology, Kumaun University, Nainital, India
3 National Institute of Malaria Research (Field Unit), Chennai, India
Correspondence Address:
Vineeta Singh
Scientist ‘C’, National Institute of Malaria Research, Sector–8, Dwarka, New Delhi–110 077
India
 Source of Support: None, Conflict of Interest: None  | Check |
PMID: 24220078 
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Background & objectives: The search for effective polymorphic markers in Plasmodium vivax is highly demanding to understand its transmission in a population. Due to the limited knowledge existing for P. vivax, the search for polymorphic markers for population studies is ongoing. The MSP gene family of Plasmodia has been linked with immune evasion. To study the circulating parasite population P. vivax merozoite surface protein 3β (PvMSP3β) polymorphic marker was used to investigate the genetic diversity of P. vivax in natural infections.
Methods: Polymorphism of PvMSP3β gene was determined in 46 P. vivax blood samples from six different regions of India by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) with PstI.
Results: Two different parasite genotypes, viz. type-A and type-B were detected among 46 samples that were positive for PCR, based on the size of the amplification. RFLP analysis with PstI showed 22 allelic groups and 15.2% samples revealed mixed infections on analysis.
Conclusion: PvMSP3β was found to be an effective molecular marker for P. vivax as it shows high diversity in India and multiple genotypes easily distinguishable without the need for sequencing. |
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